Journal
/
Chemistry
/
Methoden, um die NMR-Resonanzen von Identify<sup> 13</sup> C-Dimethyl-N-terminalen Amins auf reduktiv methylierte Proteine
JoVE Journal
Chemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Chemistry
Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins
Please note that all translations are automatically generated. Click here for the English version.

Methoden, um die NMR-Resonanzen von Identify<sup> 13</sup> C-Dimethyl-N-terminalen Amins auf reduktiv methylierte Proteine

Deutsch

Automatically Generated

6,058 Views

•

13:59 min

•

December 12, 2013

DOI:

10.3791/50875-v

DOI:
10.3791/50875-v

•

13:59 min
December 12, 2013

•

1 Views
, , ,
1Department of Chemistry,Louisiana State University

Summary

Automatically generated

Two methods for assigning the α- and ε-dimethylamine nuclear magnetic resonance signals of a reductively 13C-methylated N-terminal lysine are described. One method utilizes the pH-induced selectivity of the reductive methylation reaction, and the other uses aminopeptidase to selectively remove the N-terminal lysine.

Explore More Videos

NMRNuclear Magnetic ResonanceReductive 13C-methylation13C-dimethylamineProtein StructureProtein DynamicsMass SpectrometryLysozymeN-terminal AmineLysine Side Chain

Read Article

Cite this Article

Roberson, K. J., Brady, P. N., Sweeney, M. M., Macnaughtan, M. A. Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins. J. Vis. Exp. (82), e50875, doi:10.3791/50875 (2013).

Download .ris file

Copy

Share Video

.

Copy