JoVE Science Education
Biology I: yeast, Drosophila and C. elegans
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JoVE Science Education Biology I: yeast, Drosophila and C. elegans
Drosophila Maintenance
  • 00:00Overview
  • 00:28Nutrition
  • 01:48Housing
  • 02:31Handling
  • 04:08Anesthetizing Flies
  • 05:54Applications
  • 08:19Summary

Drosophila Maintenance

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Overview

Drosophila melanogaster, commonly known as fruit flies, are a frequently used model organism for life science research. Although starting a collection of these critters may seem as easy as leaving a banana on your kitchen counter for too long, a productive fly colony in the lab requires careful husbandry and maintenance.

This video demonstrates the necessary steps for maintaining a healthy fly stock. The overview begins with the preparation and storage of the yeast and sugar-containing media on which flies feed. Next, the vessels most commonly used for housing Drosophila are shown, as well as how and when to move flies between these containers. Finally, the presentation also includes examples of the ways in which housing and feeding conditions are manipulated for biological experiments.

Procedure

Successful Drosophila melanogaster research hinges upon correct maintenance and husbandry of this valuable model organism. The following video will discuss the necessary steps required to provide proper nutrition, housing, and handling of the fly. Though commonly know as the fruit fly, Drosophila melanogaster actually subsists on the microorganisms, such as yeast, that grow on fermenting fruit. In the lab, the fly’s diet is modified for practical purposes. Seve…

Disclosures

No conflicts of interest declared.

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JoVE Science Education Database. JoVE Science Education. Drosophila Maintenance. JoVE, Cambridge, MA, (2023).

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