Although the peripheral nervous system (PNS) is capable of significant repair after injury, little is known about the cellular and molecular mechanisms that govern this phenomenon. Using live, transgenic zebrafish and a reproducible nerve transection assay, we can study dynamic glial cell behaviors during nerve degeneration and regeneration.
Lewis, G. M., Kucenas, S. Motor Nerve Transection and Time-lapse Imaging of Glial Cell Behaviors in Live Zebrafish. J. Vis. Exp. (76), e50621, doi:10.3791/50621 (2013).