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Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells

Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells

English

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12,737 Views

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07:58 min

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June 19, 2013

DOI:

10.3791/50599-v

DOI:
10.3791/50599-v

•

07:58 min
June 19, 2013

•

3 Views
, , ,
1Department of Biology,Merrimack College, 2Center for Biotechnology and Biomedical Sciences,Merrimack College, 3Department of Technology R&D,Nexcelom Bioscience LLC

Summary

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Here, we demonstrate how image cytometry can be used for quantification of pathogenic fungi in association with host cells in culture. This technique can be used as an alternative to CFU enumeration.

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Berkes, C., Chan, L. L., Wilkinson, A., Paradis, B. Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells. J. Vis. Exp. (76), e50599, doi:10.3791/50599 (2013).

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