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Hele Mount immunfluorescensfarvning af Neonatal Mouse Retina at undersøge Angiogenesis<em> In vivo</em
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Whole Mount Immunofluorescent Staining of the Neonatal Mouse Retina to Investigate Angiogenesis In vivo
DOI:

08:47 min

July 09, 2013

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Chapters

  • 00:05Title
  • 00:56Enucleation and Fixation of Postnatal Eyes
  • 02:01Dissection of Postnatal Murine Retinas
  • 04:59Immuno/isolectin Staining of the Retinal Vasculature
  • 07:07Typical Staining of Endothelial Cell and Pericytes
  • 08:20Conclusion

Summary

Automatic Translation

Den neonatale murine nethinden giver et godt karakteriseret fysiologisk model af angiogenese, der tillader undersøgelser af de roller, som forskellige gener eller narkotika, der modulerer angiogenese i en<em> In vivo</em> Kontekst. Immunfluorescensfarvning præcist visualisere det vaskulære plexus er afgørende for succes i disse typer af undersøgelser.

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