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JoVE Journal Neuroscience
Whole Mount Immunofluorescent Staining of the Neonatal Mouse Retina to Investigate Angiogenesis In vivo
 

Whole Mount Immunofluorescent Staining of the Neonatal Mouse Retina to Investigate Angiogenesis In vivo

Article DOI: 10.3791/50546-v • 08:47 min • July 9th, 2013
July 9th, 2013 •
1, 1, 2, 1
1Institute of Genetic Medicine, Newcastle University, 2UCL Institute of Ophthalmology, University College, London

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The neonatal murine retina provides a well characterized physiological model of angiogenesis, which permits investigations of the roles of different genes or drugs that modulate angiogenesis in an in vivo context. Immunofluorescent staining to accurately visualize the vascular plexus is pivotal to the success of these types of studies.

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Whole Mount Immunofluorescent Staining Neonatal Mouse Retina Angiogenesis Blood Vessel Formation Sprouting Pre-existing Vessel Network Organ Development Tissue Development Dysregulated Blood Vessel Formation Cancer Blindness Ischemic Diseases Novel Drug Development Biological Steps Mouse Neonatal Retina Vascular Plexus Two-dimensional Structure Retinal Vascular Plexus Analysis Microscope Observation Fluorescent Staining Isolectin
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