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An Isolated Semi-intact Preparation of the Mouse Vestibular Sensory Epithelium for Electrophysiology and High-resolution Two-photon Microscopy
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Neuroscience
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JoVE Journal Neuroscience
An Isolated Semi-intact Preparation of the Mouse Vestibular Sensory Epithelium for Electrophysiology and High-resolution Two-photon Microscopy

An Isolated Semi-intact Preparation of the Mouse Vestibular Sensory Epithelium for Electrophysiology and High-resolution Two-photon Microscopy

DOI:
10.3791/50471-v

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11:52 min

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June 13, 2013

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, , , ,
1Discipline of Biomedical Science, School of Medical Sciences, Sydney Medical School,University of Sydney, 2School of Biomedical Sciences and Pharmacy,University of Newcastle

Chapters

  • 00:05Title
  • 02:23Preparation of Mouse Vestibular Sensory Epithelium
  • 06:11Electrophysiology
  • 07:44Two-photon Microscopy
  • 09:09Results: Electrophysiological Recordings and Two-photon Calcium Imaging of the Semi-intact Mouse Vestibular Sensory Epithelium
  • 11:21Conclusion

Summary

Automatic Translation

Automatic Translation

Analysis of vestibular hair cell function is complicated by their location deep within the hardest part of the skull, the petrous temporal bone. Most functional hair cell studies have used acutely isolated hair cells. Here we describe a semi-intact preparation of mouse vestibular epithelium for electrophysiological and two-photon microscopy studies.

Tags

VestibularHair CellsSensory EpitheliumElectrophysiologyTwo-photon MicroscopySemi-intact PreparationMouseCellular FunctionVestibular DysfunctionSynaptic ConnectionsSupporting Cells

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