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对糖皮质激素信号A化学筛选程序与斑马鱼幼虫的荧光素酶报告系统
JoVE Journal
Biology
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JoVE Journal Biology
A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System
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对糖皮质激素信号A化学筛选程序与斑马鱼幼虫的荧光素酶报告系统

English

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11,722 Views

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13:22 min

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September 10, 2013

DOI:

10.3791/50439-v

DOI:
10.3791/50439-v

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13:22 min
September 10, 2013

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6 Views
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1Institute of Toxicology and Genetics,Karlsruhe Institute of Technology – Campus North, 2Institute of Organic Chemistry,Karlsruhe Institute of Technology – Campus North, 3Institute of Organic Chemistry,Karlsruhe Institute of Technology – Campus South

Summary

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我们描述了利用斑马鱼幼虫糖皮质激素的应激激素信号的化学筛选系统的程序和数据分析：糖皮质激素响应在斑马鱼体内的荧光素酶活性（GRIZLY）检测。该法灵敏，特别是通过需要代谢或影响内源性糖皮质激素的生产检测化合物对糖皮质激素信号传导的影响。

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Weger, B. D., Weger, M., Jung, N., Lederer, C., Bräse, S., Dickmeis, T. A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System. J. Vis. Exp. (79), e50439, doi:10.3791/50439 (2013).

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