Dergi
/
Biyoloji
/
A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System
JoVE Journal
Biyoloji
This content is Free Access.
JoVE Journal Biyoloji
A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System

A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System

11,725 Views

•

13:22 min

•

September 10, 2013

DOI:

10.3791/50439-v

DOI:
10.3791/50439-v

•

13:22 min
September 10, 2013

•

6 Views
, , , , ,
1Institute of Toxicology and Genetics,Karlsruhe Institute of Technology – Campus North, 2Institute of Organic Chemistry,Karlsruhe Institute of Technology – Campus North, 3Institute of Organic Chemistry,Karlsruhe Institute of Technology – Campus South

Özet

Automatically generated

We describe the procedure and data analysis of a chemical screening system for glucocorticoid stress hormone signaling using zebrafish larvae: the Glucocorticoid Responsive In vivo Zebrafish Luciferase activitY (GRIZLY) assay. The assay sensitively and specifically detects effects on glucocorticoid signaling by compounds that require metabolization or affect endogenous glucocorticoid production.

Explore More Videos

Glucocorticoid SignalingZebrafish LarvaLuciferase Reporter SystemChemical ScreeningGRIZLY AssayIn VivoHigh-throughput ScreeningEndocrine DisruptorsStress Research

Read Article

Cite this Article

Weger, B. D., Weger, M., Jung, N., Lederer, C., Bräse, S., Dickmeis, T. A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System. J. Vis. Exp. (79), e50439, doi:10.3791/50439 (2013).

Download .ris file

Copy

Share Video

.

Copy