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不相关的代谢组学，生物源使用超高效液相色谱 - 高分辨质谱（UPLC-HRMS）
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JoVE Journal Chemistry
Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
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不相关的代谢组学，生物源使用超高效液相色谱 - 高分辨质谱（UPLC-HRMS）

English

Automatically Generated

22,055 Views

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11:00 min

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May 20, 2013

DOI:

10.3791/50433-v

DOI:
10.3791/50433-v

•

11:00 min
May 20, 2013

•

18 Views
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1Centers for Cancer Pharmacology and Excellence in Environmental Toxicology, Department of Pharmacology,University of Pennsylvania

Summary

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不相关的代谢组学研究提供了一个假说，产生一种代谢轮廓的快照。此协议将展示从细胞，血清或组织中的代谢产物的提取和分析。代谢物甲范围进行调查，采用液 - 液固相萃取，微流超高效液相色谱/高分辨率质谱（UPLC-HRMS）耦合到差分分析软件。

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Cite this Article

Snyder, N. W., Khezam, M., Mesaros, C. A., Worth, A., Blair, I. A. Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS). J. Vis. Exp. (75), e50433, doi:10.3791/50433 (2013).

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