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高通量清醒小鼠肾小球滤过率的测定方法

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious Mice

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高通量清醒小鼠肾小球滤过率的测定方法

42,686 Views

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07:07 min

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May 10, 2013

DOI:

10.3791/50330-v

DOI:

10.3791/50330-v

•

07:07 min

May 10, 2013

•

22 Views

Timo Rieg^1,2

¹Division of Nephrology-Hypertension, Department of Medicine,University of California, San Diego , ²San Diego VA Healthcare System

Summary

肾小球滤过率（GFR）是测量评估肾功能的金标准。在这里，我们描述了一种高通量的方法，该方法允许由一个两阶段的指数衰减模型，通过使用一个单一的大剂量注射，测定异硫氰酸荧光素（FITC）在血浆中菊粉和肾小球滤过率的计算肾小球滤过率的测定在有意识的小鼠。

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