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生きたマウスにおける血管炎症及び血栓形成の間に活性化内皮上に異型血小板、好中球の相互作用のリアルタイムイメージング
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice
DOI:

11:18 min

April 02, 2013

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Chapters

  • 00:05Title
  • 01:20Intravital Microscope and Cremaster Muscle Preparation for Intravital Microscropy
  • 02:30Intravital Microscopy for TNFα-induced Vascular Inflammation
  • 03:33Data Analysis for the Venular Inflammation Model
  • 06:06Intravital Microscopy for Laser-induced Arteriolar Thrombosis
  • 06:58Data Analysis for the Arteriolar Thrombosis Model
  • 08:20Results: Representative Heterotypic Interactions of Platelets and Neutrophils during TNFα-induced Vascular Inflammation and Platelet Thrombus at the Site of Laser-induced Arteriolar Injury in Live Mice
  • 10:52Conclusion

Summary

Automatic Translation

ここでは、血管の炎症や生きたマウスにおける血栓形成の間に活性化内皮上の異血小板、好中球の相互作用を可視化する蛍光生体顕微鏡の実験手法を報告する。この顕微鏡技術は、血管疾患の分子メカニズムを研究することが、病態生理学的な条件下での薬理学的薬剤をテストするためにも役立ちます。

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