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March 15, 2013
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1Department of Neurology, McKnight Brain Institute,University of Florida , 2Department of Entomology and Nematology,University of Florida , 3Genetics Institute, Department of Molecular Genetics and Microbiology,University of Florida , 4McKnight Brain Institute, Department of Neuroscience, Genetics Institute, Center for Translational Research on Neurodegenerative Diseases, and Center for Movement Disorders and Neurorestoration,University of Florida
Summary
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Descrevemos a seguir os processos de extracção e purificação de ARNm e metabolitos de<em> Drosophila</em> Cabeças. Estamos aplicando essas técnicas para entender melhor as perturbações celulares subjacentes degeneração neuronal. Estas metodologias podem ser facilmente dimensionados e adaptados para outras "mico" projetos.
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Jensen, K., Sanchez-Garcia, J., Williams, C., Khare, S., Mathur, K., Graze, R. M., Hahn, D. A., McIntyre, L. M., Rincon-Limas, D. E., Fernandez-Funez, P. Purification of Transcripts and Metabolites from Drosophila Heads. J. Vis. Exp. (73), e50245, doi:10.3791/50245 (2013).
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