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Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research
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Medicine
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JoVE Journal Medicine
Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

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16,010 Views

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15:18 min

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January 12, 2013

DOI:

10.3791/50238-v

DOI:
10.3791/50238-v

•

15:18 min
January 12, 2013

•

2 Views
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1Umeå Centre for Molecular Medicine,Umeå University, 2Cell Transplant Center, Diabetes Research Institute,University of Miami,, 3EMBL-CRG Systems Biology Program, Centre for Genomic Regulation,Catalan Institute of Research and Advanced Studies, 4Dept. of Computing Science,Umeå University

Summary

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We describe the adaptation of optical projection tomography (OPT)1 to imaging in the near infrared spectrum, and the implementation of a number of computational tools. These protocols enable assessments of pancreatic β-cell mass (BCM) in larger specimens, increase the multichannel capacity of the technique and increase the quality of OPT data.

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Eriksson, A. U., Svensson, C., Hörnblad, A., Cheddad, A., Kostromina, E., Eriksson, M., Norlin, N., Pileggi, A., Sharpe, J., Georgsson, F., Alanentalo, T., Ahlgren, U. Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research. J. Vis. Exp. (71), e50238, doi:10.3791/50238 (2013).

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