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מכלולי פפטיד הטרנסממברני ייצור של דיסולפיד-התייצבו ללימודים מבניים
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Chemistry
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JoVE Journal Chemistry
Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies
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מכלולי פפטיד הטרנסממברני ייצור של דיסולפיד-התייצבו ללימודים מבניים

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13,970 Views

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12:05 min

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March 06, 2013

DOI:

10.3791/50141-v

DOI:
10.3791/50141-v

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12:05 min
March 06, 2013

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1Structural Biology Division,The Walter and Eliza Hall Institute of Medical Research, 2The University of Melbourne

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Disulfide-stabilizedTransmembrane Peptide ComplexesStructural StudiesMembrane ProteinsTM DomainsTM SignalingTM Peptide ComplexesHomo-dimericHetero-dimericStable Isotope LabelsNMRNon-natural Amino AcidsDisulfide-crosslinkedDetergentLipidMembrane-mimetic

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Sharma, P., Kaywan-Lutfi, M., Krshnan, L., Byrne, E. F. X., Call, M. J., Call, M. E. Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies. J. Vis. Exp. (73), e50141, doi:10.3791/50141 (2013).

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