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Quantitative High-throughput Eencellige cytotoxiciteit T cellen
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Immunology and Infection
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JoVE Journal Immunology and Infection
Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells
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Quantitative High-throughput Eencellige cytotoxiciteit T cellen

Nederlands

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14,806 Views

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09:28 min

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February 02, 2013

DOI:

10.3791/50058-v

DOI:
10.3791/50058-v

•

09:28 min
February 02, 2013

•

4 Views
, , , ,
1Department of Chemical and Biomolecular Engineering,University of Houston , 2Division of Pediatrics, Research Unit 907,University of Texas MD Anderson Cancer Center

Summary

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We beschrijven een single-cell high-throughput assay voor cytotoxiciteit van T-cellen te meten wanneer geïncubeerd met tumor doelcellen. Deze werkwijze gebruikt een dichte, elastomeer reeks sub-nanoliter wells (~ 100.000 putjes / array) ruimtelijk beperken de T-cellen en doelcellen met gedefinieerde verhoudingen en is gekoppeld met fluorescentie microscopie effector-target conjugatie en daaropvolgende apoptose controleren.

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Cite this Article

Liadi, I., Roszik, J., Romain, G., Cooper, L. J., Varadarajan, N. Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells. J. Vis. Exp. (72), e50058, doi:10.3791/50058 (2013).

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