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Immunoprecipitation 및 아실-비오틴 거래소 (아베)에 의한 양식 Hippocampal 뉴런의 단백질 Palmitoylation의 감지
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Detection of Protein Palmitoylation in Cultured Hippocampal Neurons by Immunoprecipitation and Acyl-Biotin Exchange (ABE)
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Immunoprecipitation 및 아실-비오틴 거래소 (아베)에 의한 양식 Hippocampal 뉴런의 단백질 Palmitoylation의 감지

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34,214 Views

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16:33 min

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February 18, 2013

DOI:

10.3791/50031-v

DOI:
10.3791/50031-v

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16:33 min
February 18, 2013

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17 Views
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1Department of Cellular and Physiological Sciences, Brain Research Centre,University of British Columbia

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Protein PalmitoylationAcyl-biotin Exchange (ABE) AssayImmunoprecipitationHippocampal NeuronsProtein TraffickingPost-translational ModificationLipid ModificationCysteineHydroxylamineBiotinWestern Blotting

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Brigidi, G. S., Bamji, S. X. Detection of Protein Palmitoylation in Cultured Hippocampal Neurons by Immunoprecipitation and Acyl-Biotin Exchange (ABE). J. Vis. Exp. (72), e50031, doi:10.3791/50031 (2013).

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