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1Department of Biochemistry, Microbiology and Immunology,Neural Regeneration Laboratory and Ottawa Institute of Systems Biology, 2Department of Cellular and Molecular Medicine,University of Ottawa , 3CIHR Program in Neurodegenerative Lipidomics,University of Ottawa , 4Carleton Immersive Media Studio,Azrieli School of Architecture and Urbanism
Chapters
- 00:00Title
- 02:48Introduction
- 03:26Preparing Reagents
- 03:46Obtaining the Vaginal Smear
- 05:06Cytological Staining using Crystal Violet
- 05:46Vaginal Cytology
- 07:13Conclusion
Aqui, descrevemos como identificar o estágio da reprodução murino (proestro, estro, metaestro ou diestro) pelo simples, a coleta não-invasiva e avaliação citológica de amostras de esfregaço vaginal. Iremos descrever como citologia vaginal reflete níveis circulantes hormonais subjacentes transição durante o ciclo reprodutivo murino.
Tags
Vaginal LavageCrystal Violet StainingVaginal Cytological EvaluationMouse Estrous Cycle StagingReproductive StatusMouse Models Of DiseaseHormonal RegulationPathological ChallengeProestrusEstrusMetestrusDiestrusOvarian Steroids17-beta-estradiolProgesteroneLuteinizing HormoneFollicle Stimulating HormoneProlactinCell TypologyNucleated Epithelial CellsCornified Squamous Epithelial CellsLeukocytesVaginal SmearsInvasivenessNon-invasive Protocol
