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1Department of Biochemistry, Microbiology and Immunology,Neural Regeneration Laboratory and Ottawa Institute of Systems Biology, 2Department of Cellular and Molecular Medicine,University of Ottawa , 3CIHR Program in Neurodegenerative Lipidomics,University of Ottawa , 4Carleton Immersive Media Studio,Azrieli School of Architecture and Urbanism
Chapters
- 00:00Title
- 02:48Introduction
- 03:26Preparing Reagents
- 03:46Obtaining the Vaginal Smear
- 05:06Cytological Staining using Crystal Violet
- 05:46Vaginal Cytology
- 07:13Conclusion
Her beskriver vi, hvordan man identificerer den fase af murine reproduktive (proestrus brunst, metestrus eller diestrus) ved simpel, ikke-invasiv indsamling og cytologisk vurdering af vaginal udstrygningsprøver. Vi beskriver endvidere, hvordan vaginal cytologi afspejler cirkulerende hormonelle niveauer underliggende overgang gennem den murine reproduktive cyklus.
Tags
Vaginal LavageCrystal Violet StainingVaginal Cytological EvaluationMouse Estrous Cycle StagingReproductive StatusMouse Models Of DiseaseHormonal RegulationPathological ChallengeProestrusEstrusMetestrusDiestrusOvarian Steroids17-beta-estradiolProgesteroneLuteinizing HormoneFollicle Stimulating HormoneProlactinCell TypologyNucleated Epithelial CellsCornified Squamous Epithelial CellsLeukocytesVaginal SmearsInvasivenessNon-invasive Protocol
