/
/
Uso del mouse BLT umanizzato come cellule staminali basata su un Modello Tumore Terapia Genica
A subscription to JoVE is required to view this content. Sign in or start your free trial.
18,519 Views
•
06:59 min
•
December 18, 2012
DOI:
1Department of Medicine, Division of Hematology-Oncology,David Geffen School of Medicine at UCLA, 2UCLA AIDS Institute, 3Eli & Edythe Broad Center of Regenerative Medicine and Stem Cell Research at UCLA, 4Department of Medical and Molecular Pharmacology,David Geffen School of Medicine at UCLA, 5Department of Microbiology, Immunology and Molecular Genetics,David Geffen School of Medicine at UCLA
Summary
Automatically generated
La generazione e la caratterizzazione di cellule T specifiche tumorali usando topi umanizzati è descritto qui. Umana tessuto timico e geneticamente modificate cellule staminali ematopoietiche vengono trapiantate in topi immunocompromessi. Ciò comporta la ricostituzione di un ingegnerizzato sistema immunitario umano consentendo in esame vivo delle risposte immunitarie anti-tumorali.
Explore More Videos
BLT Humanized MouseStem Cell-based Gene TherapyTumor ModelSmall Animal ModelsMiceHuman DiseaseTherapeutic InterventionsMouse ImmunityViral DiseasesHuman Immunodeficiency Virus (HIV) InfectionHumanized Mouse ModelsC. B 17 Scid/scid MiceHuman Fetal ThymusFetal LiverThy/liv (SCID-hu)Adoptive Transfer Of Human Peripheral Blood Leukocytes (SCID-huPBL)Physiologically Relevant ModelBLT Stands For Bone Marrow/liver/thymusNOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) Immunocompromised MiceHuman Hematopoietic Stem Cell TransplantHIV Infection And Latency
Read Article
Cite this Article
Vatakis, D. N., Bristol, G. C., Kim, S. G., Levin, B., Liu, W., Radu, C. G., Kitchen, S. G., Zack, J. A. Using the BLT Humanized Mouse as a Stem Cell based Gene Therapy Tumor Model. J. Vis. Exp. (70), e4181, doi:10.3791/4181 (2012).
Copy