A subscription to JoVE is required to view this content. Sign in or start your free trial.
13,715 Views
•
11:30 min
•
December 04, 2012
DOI:
1Department of Microbiology and Immunology,Louisiana State University Health Sciences Center, 2Center for Molecular and Tumor Virology,Louisiana State University Health Sciences Center, 3Department of Microbiology and Immunology,SUNY Upstate Medical University, 4Feist-Weiller Cancer Center,Louisiana State University Health Sciences Center
Summary
Automatically generated
phagokinetic運動追跡アッセイは、細胞の動きを評価するために使用される方法です。具体的には、アッセイは、定量的な方法で時間をかけてケモキネシス（ランダム細胞の運動性）を測定します。アッセイは、コロイド金でコーティングされたカバースリップ上で彼らの動きの測定可能なトラックを作成する細胞の能力を活用しています。
Explore More Videos
Cell MigrationPhagokinetic Track Motility AssayQuantitative EvaluationCellular MotilityUnicellular OrganismsMulticellular OrganismsImmune SurveillanceWound HealingNeurological DiseasesCardiovascular DiseasesImmunological DiseasesTumor FormationActin PolymerizationReceptor RecyclingCellular ExtensionsLamellipodiaCell MotilityMicroscopyQuantitative MeasurementResource-consuming Process
Read Article
Cite this Article
Nogalski, M. T., Chan, G. C., Stevenson, E. V., Collins-McMillen, D. K., Yurochko, A. D. A Quantitative Evaluation of Cell Migration by the Phagokinetic Track Motility Assay. J. Vis. Exp. (70), e4165, doi:10.3791/4165 (2012).
Copy