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Een Parasiet Rescue and Transformation Assay voor antileishmania Screening tegen intracellulaire<em> Leishmania donovani</em> Amastigoten in THP1 Human acute monocytische leukemiecellijn
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A Parasite Rescue and Transformation Assay for Antileishmanial Screening Against Intracellular Leishmania donovani Amastigotes in THP1 Human Acute Monocytic Leukemia Cell Line
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Een Parasiet Rescue and Transformation Assay voor antileishmania Screening tegen intracellulaire<em> Leishmania donovani</em> Amastigoten in THP1 Human acute monocytische leukemiecellijn

Nederlands

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21,831 Views

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12:22 min

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December 30, 2012

DOI:

10.3791/4054-v

DOI:
10.3791/4054-v

•

12:22 min
December 30, 2012

•

7 Views
, , ,
1National Center for Natural Products Research, School of Pharmacy,University of Mississippi, 2Department of Pharmacology,University of Mississippi

Summary

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Een parasiet-rescue en transformatie test met geïnfecteerde cellen THP1<em> In vitro</em> Met<em> Leishmania donovani</em> Is geoptimaliseerd voor anti-leishmanial drug discovery. De bepaling omvat differentiatie van cellen THP1, infectie met promastigoten behandeling met testgeneesmiddelen, gecontroleerde lysis van de geïnfecteerde macrofagen, redding van amastigoten, transformatie naar promastigoten en controle promastigote groei en proliferatie met een fluorometrische assay.

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Cite this Article

Jain, S. K., Sahu, R., Walker, L. A., Tekwani, B. L. A Parasite Rescue and Transformation Assay for Antileishmanial Screening Against Intracellular Leishmania donovani Amastigotes in THP1 Human Acute Monocytic Leukemia Cell Line. J. Vis. Exp. (70), e4054, doi:10.3791/4054 (2012).

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