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Generation of an Immortalized Murine Brain Microvascular Endothelial Cell Line as an In Vitro Blood Brain Barrier Model
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Neuroscience
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JoVE Journal Neuroscience
Generation of an Immortalized Murine Brain Microvascular Endothelial Cell Line as an In Vitro Blood Brain Barrier Model

Generation of an Immortalized Murine Brain Microvascular Endothelial Cell Line as an In Vitro Blood Brain Barrier Model

DOI:
10.3791/4022-v

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09:36 min

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August 29, 2012

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, ,
1Klinik und Poliklinik für Anästhesiologie,University of Wurzburg

Chapters

  • 00:05Title
  • 01:06Isolation of Brain Microvessels
  • 04:35Immortalizing the Brain Microvascular Endothelial Cells
  • 07:22Cultivating the Brain Microvascular Endothelial Cells
  • 08:13Results: Representative Images of cEND Cells
  • 09:14Conclusion

Summary

Automatic Translation

Automatic Translation

This method describes how to isolate and immortalize microvascular endothelial cells from mouse brain. We describe a step-by-step protocol starting from the homogenization of brain tissue, digestion steps, seeding and immortalization of the cells. Usually, it takes about five weeks to obtain a homogenous, immortalized microvascular endothelial cell line.

Tags

Immortalized Cell LineMurine Brain Microvascular Endothelial CellsIn Vitro Blood Brain Barrier ModelParacellular BarriersBlood-brain Barrier (BBB)Astrocyte End-feetPericytesBasal MembraneBrain ParenchymaIn Vitro BBB ModelsPrimary Cell PreparationsImmortalized EC LinesNeonatal Mouse BrainReagents And SolutionsHomogenous Endothelial Cell LineCEND 4

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