/
/
Aislamiento y cultivo de células neuronales de ratas embrionarias: un protocolo rápido
A subscription to JoVE is required to view this content. Sign in or start your free trial.
37,631 Views
•
10:51 min
•
May 24, 2012
DOI:
Summary
Automatically generated
Se describe un método rápido para aislar y cultivar las neuronas del hipocampo y la corteza a partir de embriones de roedores. Este protocolo nos permite realizar experimentos en los que los cultivos neuronales casi puros son necesarios.
Explore More Videos
IsolationCultureRat Embryonic Neural CellsQuick ProtocolDissociationHippocampal NeuronsCortical NeuronsE15-17 Rat EmbryosMouse Primary NeuronsHuman Primary NeuronsNeural ProgenitorsSerum-free MediumNucleofectionImmunocytochemistryNucleic Acids PreparationElectrophysiologyLentiviral TransductionCalcium Phosphate TransfectionLipid-based Transfection
Read Article
Cite this Article
Pacifici, M., Peruzzi, F. Isolation and Culture of Rat Embryonic Neural Cells: A Quick Protocol. J. Vis. Exp. (63), e3965, doi:10.3791/3965 (2012).
Copy