/

Immunology and Infection

/

热酚水提取纯化及革兰氏阴性菌脂多糖的可视化

JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Immunology and Infection

Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction

Please note that all translations are automatically generated. Click here for the English version.

热酚水提取纯化及革兰氏阴性菌脂多糖的可视化

34,060 Views

•

05:31 min

•

May 28, 2012

DOI:

DOI:

•

05:31 min

May 28, 2012

•

25 Views

Michael R. Davis, Jr.¹, Joanna B. Goldberg¹

¹Department of Microbiology, Immunology, & Cancer Biology,University of Virginia Health System

Summary

我们描述一个净化革兰氏阴性菌脂多糖（LPS）的修改热水溶液中苯酚的提取方法。一旦提取，内毒素可随后通过SDS-PAGE分析，并通过可视化的直接染色或西方免疫。

Explore More Videos

Purification Visualization Lipopolysaccharide Gram-negative Bacteria Hot Aqueous-phenol Extraction Lipid A Core Oligosaccharide O-antigen Units Virulence Pathogenesis Serotyping Clinical Isolates Antibodies Mutants Extraction Method Nucleic Acids Proteins Sodium Dodecy

Read Article