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基于亲和力，外的相互作用筛选（AVEXIS）外低亲和力受体 - 配体相互作用的可扩展检测
JoVE Journal
Biology
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JoVE Journal Biology
Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions
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基于亲和力，外的相互作用筛选（AVEXIS）外低亲和力受体 - 配体相互作用的可扩展检测

English

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21,022 Views

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12:30 min

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March 05, 2012

DOI:

10.3791/3881-v

DOI:
10.3791/3881-v

•

12:30 min
March 05, 2012

•

3 Views
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1Cell Surface Signalling Laboratory,Wellcome Trust Sanger Institute

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Avidity-based Extracellular Interaction ScreeningAVEXISLow-affinity InteractionsExtracellular Receptor-ligand InteractionsIntercellular CommunicationMulticellular OrganismsProtein-protein InteractionsBinding PartnerMembrane-embedded ProteinsPosttranslational ModificationsInteraction AffinitiesHigh Throughput MethodsAVEXIS AssayWeak Protein InteractionsFalse Positive RateHigh Throughput FormatRecombinant Protein Libraries

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Kerr, J. S., Wright, G. J. Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions. J. Vis. Exp. (61), e3881, doi:10.3791/3881 (2012).

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