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September 24, 2012
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A abordagem de PCR directo apresentado aqui facilita a amplificação por PCR directamente a partir de pequenas quantidades de planta não purificada e do tecido animal.
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GenotypingPlant SamplesAnimal SamplesDNA PurificationDirect PCR ApproachThermo Scientific PhusionThermo Scientific Phire DNA PolymerasesPCR-based Target DNA DetectionPlant ResearchGenotype AnalysisTransgenesDNA IsolationThermo Scientific Phire Plant Direct PCR KitArabidopsis Plant LeavesCleaved Amplified Polymorphic Sequence Analysis (dCAPS)Single Nucleotide Polymorphisms (SNPs)Restriction Endonuclease DigestionPhenolic Compounds
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Chum, P. Y., Haimes, J. D., André, C. P., Kuusisto, P. K., Kelley, M. L. Genotyping of Plant and Animal Samples without Prior DNA Purification. J. Vis. Exp. (67), e3844, doi:10.3791/3844 (2012).
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