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Dissekering och kultur Chick Statoacoustic Ganglion och Spinal explants sladd i Collagen Gel för Neurite utväxt analyser
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Chapters
- 01:10Bead Preparation
- 01:52Statoacoustic Ganglion (SAG) Dissection
- 03:20Spinal Cord Dissection
- 05:28The Explant Culture with Either Purified Proteins or Protein-coated Beads
- 07:53Visualizing of Neurite Outgrowth by Immunohistochemistry
- 09:02Representative Results from Neurite Outgrowth Assays
- 10:42Conclusion
Vi visar hur du dissekera och kultur chick E4 statoacoustic ganglion och E6 spinal explants sladden. Explants odlas i serumfritt förhållanden i 3D kollagen geler i 24 timmar. Neurite lyhördhet testas med tillväxtfaktor-kompletterat medium och med protein-belagda pärlor.
Tags
DissectionCultureChick Statoacoustic GanglionSpinal Cord ExplantsCollagen GelsNeurite Outgrowth AssaysSensory OrgansInner EarSAG NeuronsAxon Guidance CuesSurvival FactorsOtic Axons MisroutingGrowth FactorsNeurotrophin-3 (NT-3)Brain-derived Neurotrophic Factor (BDNF)Transgenic AnimalsIn Vitro MethodResponsiveness Of SAG NeuritesSoluble ProteinsMorphogensNeuron SurvivalEmbryonic Day 4 (E4)Three-dimensional Collagen Gels
