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Dissection and Culture of Chick Statoacoustic Ganglion and Spinal Cord Explants in Collagen Gels for Neurite Outgrowth Assays
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Dissection and Culture of Chick Statoacoustic Ganglion and Spinal Cord Explants in Collagen Gels for Neurite Outgrowth Assays
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December 20, 2011
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We demonstrate how to dissect and culture chick E4 statoacoustic ganglion and E6 spinal cord explants. Explants are cultured under serum-free conditions in 3D collagen gels for 24 hours. Neurite responsiveness is tested with growth factor-supplemented medium and with protein-coated beads.
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DissectionCultureChick Statoacoustic GanglionSpinal Cord ExplantsCollagen GelsNeurite Outgrowth AssaysSensory OrgansInner EarSAG NeuronsAxon Guidance CuesSurvival FactorsOtic Axons MisroutingGrowth FactorsNeurotrophin-3 (NT-3)Brain-derived Neurotrophic Factor (BDNF)Transgenic AnimalsIn Vitro MethodResponsiveness Of SAG NeuritesSoluble ProteinsMorphogensNeuron SurvivalEmbryonic Day 4 (E4)Three-dimensional Collagen Gels
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Fantetti, K. N., Fekete, D. M. Dissection and Culture of Chick Statoacoustic Ganglion and Spinal Cord Explants in Collagen Gels for Neurite Outgrowth Assays. J. Vis. Exp. (58), e3600, doi:10.3791/3600 (2011).
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