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Whole Animal Perfusion Fixation for Rodents
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Whole Animal Perfusion Fixation for Rodents

Whole Animal Perfusion Fixation for Rodents

English

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374,484 Views

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08:53 min

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July 30, 2012

DOI:

10.3791/3564-v

DOI:
10.3791/3564-v

•

08:53 min
July 30, 2012

•

282 Views
, ,
1Biomedical Engineering,University of Michigan , 2Department of Neurological Surgery,University of Washington School of Medicine

Summary

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Here we describe a low-cost, rapid, controlled and uniform fixation procedure using 4% paraformaldehyde perfused via the vascular system: through the heart of the rat to obtain the best possible preservation of the brain.

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Cite this Article

Gage, G. J., Kipke, D. R., Shain, W. Whole Animal Perfusion Fixation for Rodents. J. Vis. Exp. (65), e3564, doi:10.3791/3564 (2012).

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