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Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation
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December 28, 2011
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Summary
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Adult-born neurons of the olfactory bulb can be optogenetically controlled using Channelrhodopsin2-expressing lentiviral injection in the rostral migratory stream and chronic photostimulation with an implanted miniature LED.
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Selective Viral TransductionAdult-born Olfactory NeuronsChronic In Vivo Optogenetic StimulationLocal InterneuronsAdult NeurogenesisLateral VentricleRostral Migratory Stream (RMS)Olfactory BulbLabel And Manipulate ActivityOptogenetic TechnologiesChannelrhodopsin2 (ChR2)-YFPTemporally Restricted Cohort Of NeuroblastsImplant And Calibrate Miniature LED
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Lepousez, G., Alonso, M., Wagner, S., Gallarda, B. W., Lledo, P. Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation. J. Vis. Exp. (58), e3380, doi:10.3791/3380 (2011).
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