Journal
/
Neuroscience
/
Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation

Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation

DOI:
10.3791/3380-v

•

12:00 min

•

December 28, 2011

•

, , , ,
1Laboratory for Perception and Memory,Institut Pasteur and Centre National de la Recherche Scientifique (CNRS)

Chapters

  • 00:05Title
  • 01:35Preparing the Microinjector
  • 02:42Preparing the Animal and Performing the Stereotaxic Injection
  • 04:53Preparing the Miniature LED for Implantation
  • 06:54Chronic LED Implantation for in vivo Optogenetic Stimulation
  • 09:29Injected Cells and Implantation
  • 10:46Conclusion

Summary

Automatic Translation

Automatic Translation

Adult-born neurons of the olfactory bulb can be optogenetically controlled using Channelrhodopsin2-expressing lentiviral injection in the rostral migratory stream and chronic photostimulation with an implanted miniature LED.

Tags

Selective Viral TransductionAdult-born Olfactory NeuronsChronic In Vivo Optogenetic StimulationLocal InterneuronsAdult NeurogenesisLateral VentricleRostral Migratory Stream (RMS)Olfactory BulbLabel And Manipulate ActivityOptogenetic TechnologiesChannelrhodopsin2 (ChR2)-YFPTemporally Restricted Cohort Of NeuroblastsImplant And Calibrate Miniature LED

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Studying the Integration of Adult-born Neurons

Lentivirus-mediated Genetic Manipulation and Visualization of Olfactory Sensory Neurons in vivo

Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons

Fiber-optic Implantation for Chronic Optogenetic Stimulation of Brain Tissue

Optogenetic Stimulation of Escape Behavior in Drosophila melanogaster

A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

Intracerebroventricular Viral Injection of the Neonatal Mouse Brain for Persistent and Widespread Neuronal Transduction

Optogenetic Stimulation of the Auditory Nerve

Ex Vivo Optogenetic Dissection of Fear Circuits in Brain Slices

Read Article