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October 25, 2011
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Here we describe a method to quantify molecular heterogeneity in histological sections of tumor material using quantitative immunofluorescence, image analysis, and a statistical measure of heterogeneity. The method is intended for use in clinical biomarker development and analysis.
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Heterogeneity MappingProtein ExpressionTumorsQuantitative ImmunofluorescenceMorphologic HeterogeneityHistopathologistsSurgical PracticePhenotype DifferencesMolecular PhenotypeProtein Expression DifferencesCellular FunctionPrognosisGenetic HeterogeneitySub-clonesMetastatic DiseaseBiomarkersTherapeutic ResponsesHistopathologic Scoring SchemesImmunohistochemistry
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Faratian, D., Christiansen, J., Gustavson, M., Jones, C., Scott, C., Um, I., Harrison, D. J. Heterogeneity Mapping of Protein Expression in Tumors using Quantitative Immunofluorescence. J. Vis. Exp. (56), e3334, doi:10.3791/3334 (2011).
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