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Derivação de Enriquecido Culturas oligodendroglial e Neuron / oligodendroglial Myelinating Co-culturas de tecidos pós-natais murino
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-cultures from Post-natal Murine Tissues

Derivação de Enriquecido Culturas oligodendroglial e Neuron / oligodendroglial Myelinating Co-culturas de tecidos pós-natais murino

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35,706 Views

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18:17 min

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August 21, 2011

DOI:

10.3791/3324-v

DOI:
10.3791/3324-v

•

18:17 min
August 21, 2011

•

4 Views
, , ,
1Regenerative Medicine Program,Ottawa Hospital Research Institute, 2Department of Cellular and Molecular Medicine,University of Ottawa , 3Department of Pharmacological Sciences,Stony Brook University, 4Department of Medicine,University of Ottawa

Summary

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Este artigo descreve métodos para derivar populações enriquecidas de células precursoras de oligodendrócitos murino (OPCs) em cultura primária, que se diferenciam para produzir oligodendrócitos maduros (MQO). Além disso, este relatório descreve as técnicas para produzir murino myelinating co-culturas semeando OPCs do mouse sobre uma cama de neurite do mouse neurônios do gânglio da raiz dorsal (DRGNs).

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O'Meara, R. W., Ryan, S. D., Colognato, H., Kothary, R. Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-cultures from Post-natal Murine Tissues. J. Vis. Exp. (54), e3324, doi:10.3791/3324 (2011).

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