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Preparação de Synaptoneurosomes de rato Cortex usando um gradiente de densidade Percoll descontínuo Sacarose-
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Neuroscience
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JoVE Journal Neuroscience
Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient

Preparação de Synaptoneurosomes de rato Cortex usando um gradiente de densidade Percoll descontínuo Sacarose-

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30,827 Views

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08:30 min

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September 17, 2011

DOI:

10.3791/3196-v

DOI:
10.3791/3196-v

•

08:30 min
September 17, 2011

•

9 Views
, , ,
1Department of Pathology and Laboratory Medicine, Waisman Center for Developmental Disabilities,University of Wisconsin, 2Department of Biochemistry, Waisman Center for Developmental Disabilities,University of Wisconsin

Summary

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Um método para preparar traducionalmente ativa, synaptoneurosomes intacta (SNs) de córtex cerebral de rato é descrito. O método utiliza um gradiente de densidade descontínua Percoll-sacarose permitindo a preparação rápida de ativos SNs.

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Westmark, P. R., Westmark, C. J., Jeevananthan, A., Malter, J. S. Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient. J. Vis. Exp. (55), e3196, doi:10.3791/3196 (2011).

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