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Packaging HIV o FIV-based Lentivector costrutti di espressione e di trasduzione del VSV-G Pseudotyped particelle virali
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April 08, 2012
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Vettori di espressione lentivirali sono i veicoli più efficaci per esprimere stabilmente diverse molecole effettrici o costrutti reporter nel dividere e non la divisione delle cellule di mammiferi e di interi organismi. Qui forniamo un protocollo su come confezionare costrutti di espressione lentivector particelle pseudoviral e di trasdurre cellule bersaglio utilizzando le particelle pseudoviral.
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HIV-based Lentivector Expression ConstructsFIV-based Lentivector Expression ConstructsTransductionVSV-G Pseudotyped Viral ParticlesPackaging Lentiviral Expression ConstructsTransfection EfficiencyTransient ExpressionPseudoviral ParticlesDifficult-to-transfect CellsPrimary CellsStem CellsDifferentiated CellsLentiviral DeliveryCellular ResponsesChemical TransfectionsTransfection Reagent ToxicityTarget CellsGenomic DNA IntegrationStable ExpressionSmall Hairpin RNA (shRNA)CDNAMicroRNAReporter GeneSelectable MarkerPuromycinGFPPseudoviral ReplicationViral Structural GenesLong Terminal Repeats (LTRs)Lentiviral Packaging
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Mendenhall, A., Lesnik, J., Mukherjee, C., Antes, T., Sengupta, R. Packaging HIV- or FIV-based Lentivector Expression Constructs & Transduction of VSV-G Pseudotyped Viral Particles. J. Vis. Exp. (62), e3171, doi:10.3791/3171 (2012).
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