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Packaging HIV o FIV-based Lentivector costrutti di espressione e di trasduzione del VSV-G Pseudotyped particelle virali
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Chapters
- 00:05Title
- 01:00Intro
- 02:28Background on Efficient Lentiviral Packaging
- 03:31Transfecting 293TN Cells with Packaging Plasmids and Expression Constructs
- 05:47Concentrating the Viral Supernatant
- 07:36Transduction of HT1080 Control Cells for Viral Titering
- 09:21Successful Transfection and Transduction
- 10:19Conclusion
Vettori di espressione lentivirali sono i veicoli più efficaci per esprimere stabilmente diverse molecole effettrici o costrutti reporter nel dividere e non la divisione delle cellule di mammiferi e di interi organismi. Qui forniamo un protocollo su come confezionare costrutti di espressione lentivector particelle pseudoviral e di trasdurre cellule bersaglio utilizzando le particelle pseudoviral.
Tags
HIV-based Lentivector Expression ConstructsFIV-based Lentivector Expression ConstructsTransductionVSV-G Pseudotyped Viral ParticlesPackaging Lentiviral Expression ConstructsTransfection EfficiencyTransient ExpressionPseudoviral ParticlesDifficult-to-transfect CellsPrimary CellsStem CellsDifferentiated CellsLentiviral DeliveryCellular ResponsesChemical TransfectionsTransfection Reagent ToxicityTarget CellsGenomic DNA IntegrationStable ExpressionSmall Hairpin RNA (shRNA)CDNAMicroRNAReporter GeneSelectable MarkerPuromycinGFPPseudoviral ReplicationViral Structural GenesLong Terminal Repeats (LTRs)Lentiviral Packaging
