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Isolering og Karakterisering af Hoechst Lav CD45 Negative Mus Lung mesenchymale stamceller
JoVE Journal
Biology
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JoVE Journal Biology
Isolation & Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells

Isolering og Karakterisering af Hoechst Lav CD45 Negative Mus Lung mesenchymale stamceller

DOI:
10.3791/3159-v

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16:55 min

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October 26, 2011

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, , , ,
1Charles C. Gates Regenerative Medicine and Stem Cell Biology Program,University of Colorado Denver, 2Department of Medicine,University of Colorado Denver, 3Cancer Center,University of Colorado Denver, 4Webb Waring Institute,University of Colorado Denver

Chapters

  • 00:05Title
  • 01:49Lung Isolation
  • 04:05Preparation of a Single Cell Suspension from Lung Tissue
  • 07:15Flow Cytometry Analysis to Define and Isolate a Lung MSC Population using Hoechst dye Efflux to Detect a Side Population
  • 13:16Enumeration of Lung MSC using a Colony Forming Assay (CFU-F)
  • 14:49Representative Analysis of Isolated MSCs
  • 16:08Conclusion

Summary

Automatic Translation

Automatic Translation

I denne artikel vil vi vise, isolering af murine bosiddende lunge mesenchymale stamceller (lunge MSC), deres ekspansion, karakterisering og analyse af immunmodulerende egenskaber.

Tags

IsolationCharacterizationHoechstlow CD45negativeMouse LungMesenchymal Stem CellsRegulatorsRegenerationFibrosisInflammationAngiogenesisTumor FormationInjuryDiseasesPulmonary FibrosisArterial HypertensionTechnologyDefine Set Of MarkersRepeated IsolationWell-characterized Stem Cell PopulationFlow CytometrySpecific Cell TypeFunction

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