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October 01, 2007
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ImmunohistochemistryIHCParaffin SectionsVectastain ABC KitVector LabsProtein ExpressionSpecific AntibodiesDirect MethodIndirect MethodPrimary AntibodiesBiotin-conjugated Secondary AntibodiesFrozen SectionsParaffin-embedded SectionsGlass SlidesDewaxingHydrationAlcohol GradientAntigen RetrievalEndogenous Peroxidase ActivityNon-specific Binding SitesT Cell Marker CD8Tyrosine HydroxylaseSecondary Antibodies Conjugated To BiotinAvidin-conjugated Horseradish Peroxidase (HRP)Diaminiobenzidine (DAB)ContrastCoverslip
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Chi, V., Chandy, K. G. Immunohistochemistry: Paraffin Sections Using the Vectastain ABC Kit from Vector Labs. J. Vis. Exp. (8), e308, doi:10.3791/308 (2007).
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