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Chapters
- 00:05Title
- 00:45In Situ Perfusion of Mouse Livers with Digestive Enzymes
- 02:37In Vitro Digestion of Mouse Livers
- 03:10Density Gradient Centrifugation
- 05:29Results of Isolating Hepatic Stellate Cells
- 06:09Conclusion
Här beskriver vi en metod för isolering av celler i levern stellate från mus lever. För stellatum cell rening, är musen lever rötas<em> På plats</em> Och<em> In vitro</em> Genom pronase-kollagenas behandling före densitetsgradient centrifugering. Denna teknik ger mycket ren celler nedsatt stellate.
Tags
Stellate CellsHepatic Stellate CellsLiver-resident CellsSpace Of DisseBone Marrow PrecursorsVitamin A StorageMyofibroblastsExtracellular MatrixLiver FibrosisPortal HypertensionAntigen Presenting CellsNKT CellsT LymphocytesIsolation Of Stellate CellsPerisinusoidal LocalizationDigestive EnzymesPronase ECollagenase PIn Vitro Enzymatic TreatmentVitamin A-storing Lipid DropletsCentrifugation
