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使用全山原位杂交分子和有机体生物学链接

JoVE Journal
Biology

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JoVE Journal Biology

Using Whole Mount in situ Hybridization to Link Molecular and Organismal Biology

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使用全山原位杂交分子和有机体生物学链接

DOI:

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12:50 min

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March 31, 2011

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Nicole L. Jacobs, R. Craig Albertson, Jason R. Wiles²

¹Department of Biology,Syracuse University, ²Department of Science Teaching,Syracuse University

Chapters

00:05Title
02:04Fixation of Embryos and Proteinase K Digestion
05:34Hybridization of Riboprobes
07:42Blocking and Anti-Digoxigenin (alpha-DIG) Antibody Incubation
09:11Staining and Final Processing
10:58Expression of aldh1a2, fgf8a, and pax2a in Zebrafish Embryos
12:12Conclusion

Summary

Automatic Translation

整装<em>原位</em>杂交（拟）是用于高层本科比较脊椎动物生物学除了脊椎动物解剖课程。这使学生有机会来研究基因表达模式以及大体解剖，连接分子和有机体生物学研究的一门课程内。

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