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Immunology and Infection
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RNA Interference in Ticks
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
RNA Interference in Ticks

RNA Interference in Ticks

English

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17,778 Views

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09:06 min

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January 20, 2011

DOI:

10.3791/2474-v

DOI:
10.3791/2474-v

•

09:06 min
January 20, 2011

•

1 Views
, ,
1Department of Veterinary Pathobiology, Center for Veterinary Health Sciences,Oklahoma State University, 2(CSIC-UCLM-JCCM),Instituto de Investigación en Recursos Cinegéticos IREC

Summary

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A method for RNA interference (RNAi) by injection of dsRNA into unfed ticks is described. RNAi is the most widely used gene-silencing technique in ticks where the use of other methods of genetic manipulation has been limited.

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RNA InterferenceTicksHematophagous EctoparasitesVectors Of Human DiseasesCattle IndustryControl Tick InfestationsRNAiGene Expression DisruptionSmall Interfering RNAs (siRNAs)Double-stranded RNA (dsRNA)Post-transcriptional Gene SilencingFunctional Genomics StudiesGenetic Manipulation

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Cite this Article

Kocan, K. M., Blouin, E., de la Fuente, J. RNA Interference in Ticks. J. Vis. Exp. (47), e2474, doi:10.3791/2474 (2011).

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