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Pseudomonas aeruginosa e Saccharomyces cerevisiae Biofilme em Células de Fluxo
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January 15, 2011
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Protocolo que descreve a aplicação de um sistema de célula de fluxo para o cultivo e análise de biofilmes microbianos para Microscopia Confocal Laser Scanning (CLSM).
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Pseudomonas AeruginosaSaccharomyces CerevisiaeBiofilmFlow CellsMicrobial CommunitiesAltered Physiological PropertiesPathological PropertiesTransparent SubstratumNon-destructive ExaminationReal-time StudiesFlow Cell Model SystemReproducibilityGrowth ChamberNutrientsOxygen SupplyPeristaltic PumpWaste ContainerContinuous Supply Of NutrientsAdministration Of AntibioticsMinimal DisturbanceShear StressBubble Trapping DeviceConfocal Laser Scanning Microscopy (CLSM)3D Information
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Weiss Nielsen, M., Sternberg, C., Molin, S., Regenberg, B. Pseudomonas aeruginosa and Saccharomyces cerevisiae Biofilm in Flow Cells. J. Vis. Exp. (47), e2383, doi:10.3791/2383 (2011).
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