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Chapters
- 00:00Title
- 00:57Introduction
- 01:58Preparing Anti-PECAM-1 Antibody-Conjugated Magnetic Beads
- 03:36Isolating Mouse Pulmonary Endothelial Cells from Neonatal Mice
- 06:56Sorting Mouse Lung Endothelial Cells with Anti-ICAM-2 Dynabeads
- 09:13Evaluation of Pulmonary Endothelial Cells from Neonatal Mice
- 09:57Conclusion
Tags
IsolationCulturePulmonary Endothelial CellsNeonatal MiceResearch ModelVascular BiologyHuman Umbilical Vein Endothelial Cells (HUVECs)Microvascular Endothelial Cells (ECs)AngiogenesisPermeabilityKnockout MiceProtein Function AnalysisIsolation ProtocolCulturing ProtocolMouse Lung Endothelial Cells (MLECs)Collagenase/dispase (C/D) SolutionSingle-cell SuspensionPositive SelectionAnti-PECAM-1 AntibodyDynabeadsMagnetic Particle Concentrator (MPC)
