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Journal
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Immunology and Infection
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羧基荧光素二醋酸琥珀酰亚胺酯（CFSE）用于监视淋巴细胞增殖
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
The Use of Carboxyfluorescein Diacetate Succinimidyl Ester (CFSE) to Monitor Lymphocyte Proliferation
Please note that all translations are automatically generated.
Click here for the English version.
羧基荧光素二醋酸琥珀酰亚胺酯（CFSE）用于监视淋巴细胞增殖
DOI:
10.3791/2259-v
•
04:10 min
•
October 12, 2010
•
Benjamin J. C. Quah
,
Christopher R. Parish
1
Department of Immunology
,
John Curtin School of Medical Research, Australian National University
Chapters
00:00
Title
01:16
CFSE Labeling
03:01
The CFSE Method Used
in vitro
and
in vivo
03:50
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
CFSE共价键的标签与荧光染料，羧基的长寿命的细胞内分子。因此，一个CFSE标记的细胞分裂时，它的后代有一半的荧光量，从而可以被用来评估细胞分裂。本文介绍与CFSE标记的小鼠淋巴细胞通常使用的程序。
Tags
Carboxyfluorescein Diacetate Succinimidyl Ester
CFSE
Lymphocyte Proliferation
Fluorescent Dye
Carboxyfluorescein
Cell Division
Flow Cytometry
Labeling Mouse Lymphocytes
Multi-color Flow Cytometry
In Vitro Studies
In Vivo Studies
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