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Differentiating Non-Transgenic and Transgenic Human Pluripotent Stem Cells into Neural Progenitor Cells
Transcript
For the generation of non-transgenic neuronal progenitors and neurons, or hNeurons, see day 14 hPSC-derived neural progenitors into ECM-coated six-well plates with 2 milliliters of neural medium and Y27632 per well.
If working with doxycycline-inducible iNeurons, add neural medium containing doxycycline when the cells are about 35% confluent to induce differentiation and activate gene expression. After two days, feed transgenic and non-transgenic lines with 2 milliliters of neural media per well per day. Continue until the cells are ready to lift at 70% confluency.
