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Marcado específico del VIH-1 células positivas utilizando un vector lentiviral Ap-dependientes que expresan la proteína verde fluorescente
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September 23, 2010
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HIV-1Marking HIV-positive CellsRev-dependent Lentiviral VectorGreen Fluorescent Protein (GFP)HIV-responsive Expression VectorsLong Terminal Repeat (LTR)Tat-responsive LTRCellular Activation StatesLocal Chromatin ActivityHIV-independent ActivityLTR-based ReporterHIV DNA SequencesRev-response ElementHIV Splicing SitesLentiviral Reporter VirusReplicating HIVLiving Cell PopulationsGreen Fluorescence Protein (GFP) ExpressionIn Situ PCRHIV Antigen StainingTherapeutic Genes
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Guo, J., Enos, C., Wu, Y. Specific Marking of HIV-1 Positive Cells using a Rev-dependent Lentiviral Vector Expressing the Green Fluorescent Protein. J. Vis. Exp. (43), e2198, doi:10.3791/2198 (2010).
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