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Chapters
- 00:00Title
- 01:06Introduction
- 01:44Site Directed Mutagenesis and Preparation of Plasmid DNA for Transfection
- 03:09Plating of Cells, Transient Transfection, and Imaging of Transfected Cells
- 05:00Whole-cell Patch Clamping
- 12:57Representative Analysis
- 14:42Conclusion
Tags
MutagenesisFunctional AnalysisIon ChannelsHeterologously ExpressedMammalian CellsPoint MutationGIRK ChannelsG Protein-gated Inwardly Rectifying Potassium ChannelsExcitability Of NeuronsG Protein-coupled ReceptorsMuscarinic ReceptorAlcohol ActivationAmino Acid MutationCDNA SequencingTransfectionHEK293T CellsPatch-clamp ElectrophysiologyPotassium CurrentsL257W Mutation
