Journal
/
Neuroscience
/
No útero Eletroporação seguido por Cultura Neuronal primário para estudar a função do gene no subconjunto dos neurônios corticais
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons

No útero Eletroporação seguido por Cultura Neuronal primário para estudar a função do gene no subconjunto dos neurônios corticais

português

Automatically Generated

17,489 Views

•

08:24 min

•

October 08, 2010

DOI:

10.3791/2103-v

DOI:
10.3791/2103-v

•

08:24 min
October 08, 2010

•

12 Views
, , , ,
1Center for Neurologic Diseases,Brigham and Woman’s Hospital and Harvard Medical School, 2Department of Physiology and Neurobiology,University of Connecticut

Explore More Videos

In Utero ElectroporationPrimary Neuronal CultureGene FunctionSubset Of Cortical NeuronsNeurite OutgrowthGenetic AlterationsShRNACDNA ConstructsChemical TransfectionViral TransductionHeterogeneous Pool Of Cell TypesGlutamatergic NeuronsInhibitory NeuronsGlial CellsEmbryonic Rodent CortexDeep Layers Of The CortexSuperficial Layers Of The CortexDorsal-medial Regions Of The CortexVentral-lateral Regions Of The CortexQuantitative AnalysisStep-by-step Method

Read Article

Cite this Article

Rice, H., Suth, S., Cavanaugh, W., Bai, J., Young-Pearse, T. L. In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons. J. Vis. Exp. (44), e2103, doi:10.3791/2103 (2010).

Download .ris file

Copy

Share Video

.

Copy