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September 03, 2010
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Laser ablation electrospray ionization (LAESI) is an atmospheric-pressure ion source for mass spectrometry. In the imaging mode, a mid-infrared laser probes the distributions of molecules across a tissue section or a biofilm. This technique presents a new approach for diverse bioanalytical studies carried out under native experimental conditions.
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Atmospheric-pressure Molecular ImagingBiofilmsLAESI Mass SpectrometryAmbient Ionization MethodsLaser Ablation Electrospray IonizationWater-containing SpecimensMid-infrared Laser BeamAblation Fluence ThresholdParticulate MatterAblation PlumeHighly Charged DropletsGas-phase IonsMass SpectrometerAtmospheric-pressure Ion Source InterfaceMolecular ImagingMicroprobe Analysis ModeDepth ProfilingLateral ImagingThree-dimensional (3D) Molecular ImagingLateral ResolutionDepth Resolution
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Nemes, P., Vertes, A. Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry. J. Vis. Exp. (43), e2097, doi:10.3791/2097 (2010).
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