Intermittent Photic Stimulation in Rabbit Model: A Technique to Induce Epileptic Seizures and Simultaneously Record Multisystem Parameters
Transcript
Photic stimulation is a technique where intermittent light flashes elicit a seizure response in epilepsy patients. Since the cardiac activity of rabbits is comparable to that of humans, they are an ideal model for epilepsy research.
Begin with a rabbit secured in a restrainer with EEG and ECG electrodes attached. A restrainer helps acquire readings in a conscious state, omitting the need for anesthesia. Attach a pulse oximeter to the rabbit's ear and mask with capnography tubing over its mouth. These instruments capture respiratory activity to continuously assess oxygenation and ventilation.
Next, place an appropriate light source at the rabbit's eye level. Use mirrors on either side of and behind the rabbit's head to ensure light enters its eyes. Now, flash the light of a low frequency and maximum intensity. Expose the rabbit to light with its eyes open. Then cover the rabbit's face to simulate eye closure, triggering photosensitivity to seizures.
Repeat the experiment by gradually increasing the frequency and then decreasing it. Record the corresponding changes in cerebral and cardiac functions using video EEG and ECG techniques. Subsequently, remove the electrodes and return the rabbit to its home cage for routine care.
