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在分离的小鼠使用红桃心功能和能量学的评估 31 P核磁共振
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal Medicine
Assessment of Cardiac Function and Energetics in Isolated Mouse Hearts Using 31P NMR Spectroscopy
DOI:

11:38 min

August 31, 2010

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Chapters

  • 00:00Title
  • 00:59Introduction
  • 01:25Experimental Set-up
  • 04:33Isolated Heart Preparation
  • 07:41Assessment of Cardiac Function and Energetics Using 31P NMR Spectroscopy
  • 09:43Representative Results of Cardiac Function and Energetics in Isolated Mouse Hearts Using 31P NMR Spectroscopy
  • 11:09Conclusion

Summary

Automatic Translation

离体心脏灌流的Langendorff -模式,配合<sup> 31</sup> P核磁共振光谱,结合成一个实验生物化学和生理学等领域。该协议允许动态测量高能磷酸含量和心的同时监测生理功能的营业额。当正确执行,这是一个有价值的技术评估心脏能量。

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